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From a genomic target to PCR primers.

Take the next step from exploring a gene to planning an experiment. Generate candidate primer pairs for your selected genomic locations, with their supporting checks in one workbench.

Open primer workbench Sign in to start designing · Powered by Primer3

How primer design works

YOUR TARGET, IN FOCUSPCR DESIGN
A primer pair flanking a genomic target Forward and reverse primers point inward on opposite DNA strands, surrounding the selected target and defining an amplicon. This is an illustration, not a computed design. Selected target 5′3′ 3′5′ Forward Reverse Amplicon
Illustrative workflow · your target defines the design.
SpecificityThermodynamicsProduct size
  1. 01

    Select your target

    Choose a genomic location and a PCR preset.

  2. 02

    Generate pairs

    Use Primer3 to design candidate forward and reverse primers.

  3. 03

    Review the checks

    Inspect specificity, thermodynamics, and the expected product.

  4. 04

    Save and export

    Keep a primer library and export CSV, FASTA, or JSON.

Computed candidates support experiment planning. Validate primer performance in the lab. This describes genomic PCR design, not qPCR or probe design.

YOUR NEXT QUESTION STARTS HERE

There's more in your genome.
Let's explore it with Ely.

Your first 5 strains free · account activation reviewed

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